You used headspace sampling but also mentioned HiSorb™ is compatible with immersive sampling, how does this work? All the robotic techniques, like headspace and SPME, can be used with the focusing trap to enhance the quality of information obtained from every sample. There is also a tube desorption module, so you can analyze sorbent pack tubes or tubes with samples placed within them for direct desorption. With robotic automation, you can also perform syringe-based headspace, solid-phase microextraction (SPME), and liquid handling as well. RS: HiSorb TM is one of many techniques that are available on the Centri ® platform.
Is HiSorb™ the only technique compatible with the Centri ® platform? It then automatically inserts the probes for sample extraction. We prepare the vials and load them onto the sample tray and the Centri® system automatically collects the vials and heats them. Is the sampling from the headspace automated?ĪM: With the Centri ® platform, sampling is fully automated. This way we can make sure that any sort of changes in biomarker signal is related to the samples and not instrument performance. Do you use an internal standard during the sample preparation to account for the differing HiSorb™ probes you've used in the study?ĪM: We add internal standards, such as deuterated standards, to samples so that we can monitor instrument performance and account for any drops in signal during the measurement. They're not a biosensor-based device, instead we are looking for those biomarker signals with GC-MS. Does this method use a biosensor-based device?ĪM: We use devices for collecting breath samples and then transfer these to analytical instruments.
So, something that might look promising from the culture experiment might be even better carried out on a breath sample because these samples are more stable. Whereas the temperature and pH typical of the human body mean that some of these probes are a lot more stable and so we observe less of a loss of analyte due to evaporation than we might see in the culture. We found that the temperature we incubate the culture samples at is more likely to lead to a loss of limonene from evaporation. What is the effect of body temperature and pH on the detection of biomarkers from breath samples?ĪM: To determine the effects of temperature and pH on detection, we compared the extraction of limonene from the culture samples versus the results with limonene extracted from the breath samples. Read on for highlights of the live Q&A session or register now to watch the webinar at any time that suits you.
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